Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5217] to KAP1
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-KAP1 antibody [EPR5217]
See all KAP1 primary antibodies
Description

Rabbit monoclonal [EPR5217] to KAP1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues on the C-terminus in Human KAP1.
Positive control
- Purchase matching WB positive control:Recombinant Human KAP1 protein
- A431, HeLa, PC-3, and F9 cell lysates, Human spleen tissue, HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5217
Isotype

IgG
Research areas
- Cancer
- Cell Death
- Apoptosis
- Other
- Epigenetics and Nuclear Signaling
- Bromodomains

Western blot - Anti-KAP1 antibody [EPR5217] (ab109289)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Wild type HAP1 + DMSO whole cell lysate (20 µg)
Lane 3: Wild type HAP1 + Blaomycin whole cell lysate (20 µg)
Lane 4: TRIM28 knockout HAP1 whole cell lysate (20 µg)
Lane 5: TRIM28 knockout HAP1 + DMSO whole cell lysate (20 µg)
Lane 6: TRIM28 knockout HAP1 + Blaomycin whole cell lysate (20 µg)
Lane 7: HeLa + DMSO whole cell lysate (20 µg)
Lane 8: Hela + Blaomycin whole cell lysate (20 µg)

Lanes 1 - 8: Merged signal (red and green). Green - ab109289 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109289 was shown to specifically react with KAP1 in wild type cells as signal was lost in KAP1 knockout cells. Wild-type and KAP1 knockout samples were subjected to SDS-PAGE. Ab109289 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-KAP1 antibody [EPR5217] (ab109289)

All lanes : Anti-KAP1 antibody [EPR5217] (ab109289) at 1/1000 dilution

Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : F9 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 89 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5217] (ab109289)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue using ab109289 at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody [EPR5217] (ab109289)

Immunofluorescent staining of HeLa cells using ab109289 at a dilution of 1/100.
Anti-KAP1 antibody [EPR5217] (ab109289)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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