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Human CBX3 (HP1 gamma) knockout HeLa cell pellet (ab278874)

Overview

  • Product name

    Human CBX3 (HP1 gamma) knockout HeLa cell pellet
    See all HP1 gamma/CBX3 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon 2 and 5 bp deletion in exon 2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

  • Sequence similarities

    Contains 2 chromo domains.

  • Post-translational
    modifications

    Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

  • Cellular localization

    Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
  • Information by UniProt

  • Alternative names

    • CBX 3
    • CBX3
    • CBX3_HUMAN
    • Chromobox homolog 3
    • Chromobox homolog 3 (HP1 gamma homolog, Drosophila)
    • Chromobox protein homolog 3
    • GAMMA
    • HECH
    • Heterochromatin like protein 1
    • Heterochromatin protein 1 homolog gamma
    • Heterochromatin protein HP1 gamma
    • HP1 gamma
    • HP1 gamma homolog
    • HP1Hs gamma
    • Modifier 2 protein
    see all

Properties

Images

  • Western blot - Human CBX3 (HP1 gamma) knockout HeLa cell pellet (ab278874)
    Western blot - Human CBX3 (HP1 gamma) knockout HeLa cell pellet (ab278874)
    Lane 1: Wild-type HeLa cell lysate (20µg)

    Lane 2: CBX3 knockout HeLa cell lysate (20µg)
    Lanes 1- 2: Merged signal (red and green). Green - ab217999 observed at 25 kDa. Red - loading control ab7291 observed at 50 kDa.
    ab217999 Anti-HP1 gamma/CBX3 antibody [EPR19802] was shown to specifically react with HP1 gamma/CBX3 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261744 (knockout cell lysate ab257110) was used. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab217999 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Sanger Sequencing - Human CBX3 (HP1 gamma) knockout HeLa cell pellet (ab278874)
    Sanger Sequencing - Human CBX3 (HP1 gamma) knockout HeLa cell pellet (ab278874)

    Allele-1: 5 bp deletion in exon 2

     

  • Sanger Sequencing - Human CBX3 (HP1 gamma) knockout HeLa cell pellet (ab278874)
    Sanger Sequencing - Human CBX3 (HP1 gamma) knockout HeLa cell pellet (ab278874)

    Allele-2: 4 bp deletion in exon 2

     

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