Fluconazole, Cytochrome P450 inhibitor (ab141065)
Key features and details
- Triazole antifungal agent. Cytochrome P450 inhibitor.
- CAS Number: 86386-73-4
- Purity: > 99%
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Fluconazole, Cytochrome P450 inhibitor -
Description
Triazole antifungal agent. Cytochrome P450 inhibitor. -
Biological description
Triazole antifungal agent. Cytochrome P450 inhibitor (IC50 values are 30.3, 12.3, 13.1 μM for CYP2C9, CYP2C19 and CYP3A4, respectively). Penetrates the blood-brain barrier.
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Purity
> 99% -
CAS Number
86386-73-4 -
Chemical structure
Properties
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Chemical name
2-(2,4-Difluorophenyl)-1,3-bis(1H-1,2,4-triazol-1-yl)propan-2-ol -
Molecular weight
306.27 -
Molecular formula
C13H12F2N6O -
PubChem identifier
3365 -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
OC(Cn1cncn1)(Cn2cncn2)c3ccc(F)cc3F -
Source
Synthetic
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Research areas
Images
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Functional Studies - Fluconazole, Cytochrome P450 inhibitor (ab141065)
HepG2 cells were incubated at 37ºC for 24h with vehicle control (0 µM) and 100 µM of fluconazole (ab141065). Increased expression of cytochrome P450 1A1 (ab3568) correlates with an increase in fluconazole concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3568 at 1/500 dilution and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
