Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 45 min
- Sample type: Adherent cells, Plasma, Saliva, Serum, Suspension cells, Tissue, Urine
- Sensitivity: 40 pg/well
Overview
-
Product name
Hydroxymethylated DNA Quantification Kit (Colorimetric)
See all hydroxymethylated DNA kits -
Detection method
Colorimetric -
Sample type
Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
>= 40 pg/well -
Assay time
3h 45m -
Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.
Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.
It can be paired to use with Methylated DNA Quantification Kit (Colorimetric) (ab117128), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.
-
Platform
Microplate reader
Properties
-
Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Binding Solution 1 x 5ml 1 x 10ml Capture Antibody, 1000 µg/mL 1 x 4µl 1 x 8µl Detection Antibody, 400 µg/mL 1 x 8µl 1 x 16µl Developer Solution 1 x 5ml 1 x 10ml Enhancer Solution 1 x 8µl 1 x 16µl Negative Control I, 20 µg/mL 1 x 10µl 1 x 20µl Negative Control II, 20 µg/mL 1 x 10µl 1 x 20µl Positive Control, 20 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 5ml 1 x 10ml -
Research areas
Images
-
Functional Studies - Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130)Demonstration of a high abundance of 5-hydroxymethylcytosine in human brain and colon tissues. 200 ng of DNA isolated from different tissues or cells were added into the assay wells and the hydroxymethylated fraction of DNA was measured using the ab117130. OD intensity measured in variety of DNA samples.
-
Functional Studies - Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130)Demonstration of high specificity of 5-hydroxymethylcytosine detection achieved using ab117130. Synthetic unmethylated DNA (contains only cytosine), methylated DNA (contains only 5-methylcytosine), and hydroxymethylated DNA standard (contains only 5-hydroxymethylcytosine) were added into the assay wells at different concentrations and then measured with ab117130. -
Functional Studies - Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130)Demonstration of a high abundance of 5-hydroxymethylcytosine in human brain and colon tissues. 200 ng of DNA isolated from different tissues or cells were added into the assay wells and the hydroxymethylated fraction of DNA was measured using ab117130. Percentage of hydroxymethylated DNA in various tissues and cells. -
Functional Studies - Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130)Demonstration of high sensitivity of 5-hydroxymethylcytosine detection achieved using ab117130. Synthetic hydroxymethylated DNA was added into the assay wells at different concentrations and then measured with the ab117130.
