Mouse MIP1b ELISA Kit (ab215115)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.1 pg/ml
- Range: 5 pg/ml - 350 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MIP1b ELISA Kit
See all CCL4/MIP-1 beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 5% Inter-assay Sample n Mean SD CV% Overall 3 2.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.1 pg/ml -
Range
5 pg/ml - 350 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 111 105% - 117% Serum 101 94% - 110% Hep Plasma 81 76% - 89% EDTA Plasma 90 87% - 93% Cit plasma 81 77% - 84% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse MIP1b ELISA Kit (ab215115) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP1b protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MIP1b with 2.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MIP1b (CCL4) is a 7.8 kDa protein member of the CC subfamily of chemokines. MIP1b is induced in a variety of cells, including mast cells, CD8+ T cells, intra-epithelial gamma-delta T cells, endothelial cells, and macrophages. MIP1b is involved in the recruitment of T cells to lymph nodes and can enhance macrophage effector functions.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml 10X Mouse MIP1b Capture Antibody 1 x 600µl 10X Mouse MIP1b Detector Antibody 1 x 600µl Mouse MIP1b Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all -
Database links
- Entrez Gene: 20303 Mouse
- SwissProt: P14097 Mouse
- Unigene: 244263 Mouse
Images
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Other - Mouse MIP1b ELISA Kit (ab215115)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse MIP1b standard curve comparison data.Standard curve comparison between mouse MIP1b SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 0.5-fold increase in sensitivity.
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Example of mouse MIP1b standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MIP1b in cell culture supernatant sample.The concentrations of MIP1b were measured in duplicates, interpolated from the MIP1b standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW264.7 (+LPS, +IFNg) supernatant 75%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1b concentration was determined to be 441 pg/mL.
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Native pooled mouse serum and plasma signal was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1b concentration was determined to be 89 pg/m/l in serum, 26 pg/mL in plasma (citrate), 28 pg/mL in plasma (heparin), 31 pg/mL in plasma (EDTA).
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Sandwich ELISA - Mouse MIP1b ELISA Kit (ab215115)To learn more about the advantages of recombinant antibodies see here.
