Human CCL23 Matched Antibody Pair Kit (ab218806)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Human CCL23 Matched Antibody Pair Kit
See all CCL23 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.7 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human CCL23 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human CCL23.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216169. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human CCL23 Capture Antibody 2 x 50µg 1 x 50µg Human CCL23 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human CCL23 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. -
Tissue specificity
High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine ligand 23
- C-C motif chemokine 23
- C6 beta chemokine
see all -
Database links
- Entrez Gene: 6368 Human
- Omim: 602494 Human
- SwissProt: P55773 Human
- Unigene: 169191 Human
Images
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Human MIP3 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human CCL23 Matched Antibody Pair Kit (ab218806)To learn more about the advantages of recombinant antibodies see here.
