Human CCL23 ELISA Kit (ab216169)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.54 pg/ml
- Range: 7.03 pg/ml - 450 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CCL23 ELISA Kit
See all CCL23 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 8.4% Inter-assay Sample n Mean SD CV% Serum 3 5.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.54 pg/ml -
Range
7.03 pg/ml - 450 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 91 89% - 92% Serum 106 98% - 117% Other biological fluids 93 88% - 96% Hep Plasma 98 88% - 106% EDTA Plasma 101 98% - 106% Cit plasma 105 93% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CCL23 ELISA Kit (ab216169) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL23 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL23 with 1.54 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CCL23 (Macrophage inflammatory protein 3) is a chemokine involved in the recruitment of monocytes, neutrophils and resting T cells. CCL23 functions by inhibition myeloid progenitor cell proliferation. CCL23 is found at high levels in the synovial fluid of patients with rheumatoid arthritis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CCL23 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human CCL23 Capture Antibody (Lyophilized) 1 vial Human CCL23 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. -
Tissue specificity
High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine ligand 23
- C-C motif chemokine 23
- C6 beta chemokine
see all -
Database links
- Entrez Gene: 6368 Human
- Omim: 602494 Human
- SwissProt: P55773 Human
- Unigene: 169191 Human
Images
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Other - Human CCL23 ELISA Kit (ab216169)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human MIP3 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MIP3 in human serum and plasma samples.The concentrations of MIP3 were measured in duplicates, interpolated from the MIP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 698 pg/mL in serum, 621 pg/mL in plasma (citrate), 689 pg/mL in plasma (heparin) and 592 pg/mL in plasma (EDTA).
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Interpolated concentrations of native MIP3 in human synovial fluid and THP-1 cell culture supernatant samplesThe concentrations of MIP3 were measured in duplicates, interpolated from the MIP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: synovial fluid 25% and THP-1 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 182 pg/mL in synovial fluid and 130 pg/mL in THP-1 supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 797 pg/mL with a range of 229 – 1,754 pg/mL.
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Sandwich ELISA - Human CCL23 ELISA Kit (ab216169)To learn more about the advantages of recombinant antibodies see here.
