Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 3 hr 20 min
- Sample type: Adherent cells, Plasma, Saliva, Suspension cells, Tissue, Urine
- Sensitivity: 10 pg/well
Overview
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Product name
Hydroxymethylated DNA Quantification Kit (Fluorometric)
See all hydroxymethylated DNA kits -
Detection method
Fluorescent -
Sample type
Saliva, Urine, Plasma, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
>= 10 pg/well -
Assay time
3h 20m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.
Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.
It can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric) (ab117129), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Binding Solution 1 x 5ml 1 x 10ml Capture Antibody, 1000 µg/mL 1 x 4µl 1 x 8µl Detection Antibody, 400 µg/mL 1 x 8µl 1 x 16µl Enhancer Solution 1 x 8µl 1 x 16µl Fluoro Developer 1 x 8µl 1 x 16µl Fluoro Dilutor 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 8µl 1 x 16µl Negative Control I, 20 µg/mL 1 x 10µl 1 x 20µl Negative Control II, 20 µg/mL 1 x 10µl 1 x 20µl Positive Control, 20 µg/mL 1 x 10µl 1 x 20µl -
Research areas
Images
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Functional Studies - Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131)Demonstration of high sensitivity and specificity of 5-hydroxymethylcytosine detection achieved using ab117131. Synthetic unmethylated DNA (contains only cytosine), methylated DNA (contains only 5-methylcytosine), and hydroxymethylated DNA standard (contains only 5-hydroxymethylcytosine) were added into the assay wells at different concentrations and then measured with ab117131.
