Anti-CBX4 antibody (ab4189)
Key features and details
- Rabbit polyclonal to CBX4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CBX4 antibody
See all CBX4 primary antibodies -
Description
Rabbit polyclonal to CBX4 -
Host species
Rabbit -
Specificity
In immunofluoresence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localisation of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see picture). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody also reacts with another ubiquitous highly expressed protein. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide:
GLQDSSTDNRAKLC
, corresponding to amino acids 95 - 107 of Human PC2. -
General notes
PC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. -
Primary antibody notes
PC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CBX4 antibody (ab4189) This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK
Immunofluorescent imaging of human cells (U2OS) with ab4189 reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localisation of PC2. This corresponds to results seen in Satijn et al.
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees C. Nuclei counterstained with Hoechst stain (blue).
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Western blot - Anti-CBX4 antibody (ab4189) This image is courtesy of Dr Ari Melnick, Albert Einstein College of MedicineAll lanes : Anti-CBX4 antibody (ab4189)
Lane 1 : HEK 293 lysate over-expressing DDDDK tagged hPC2
Lane 2 : HEK 293 lysate
Predicted band size: 61 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible non-specific secondary antibody binding), 49 kDa (possible non-specific secondary antibody binding), 65 kDa (possible non-specific secondary antibody binding)
ab4189 specifically recognises over expressed DDDDK tagged hPC2 in HEK293 cells (lane1). The lower bands are assigned to degradation products.
