Methylated DNA Quantification Kit (Colorimetric) (ab117128)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 4 hr
- Sample type: Adherent cells, Plasma, Saliva, Serum, Suspension cells, Tissue, Urine
- Sensitivity: 0.2 ng/well
Overview
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Product name
Methylated DNA Quantification Kit (Colorimetric)
See all 5-methylcytosine kits -
Detection method
Colorimetric -
Sample type
Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
>= 0.2 ng/well -
Assay time
4h 00m -
Species reactivity
Reacts with: Species independent -
Product overview
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.
Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
It can be paired to use with Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Binding Solution 1 x 5ml 1 x 10ml Capture Antibody, 1000 µg/ml 1 x 4µl 1 x 8µl Detection Antibody, 400 µg/ml 1 x 8µl 1 x 16µl Developer Solution 1 x 5ml 1 x 10ml Enhancer Solution 1 x 8µl 1 x 16µl Negative Control, 20 µg/ml 1 x 10µl 1 x 20µl Positive Control, 20 µg/ml 1 x 10µl 1 x 20µl Stop Solution 1 x 5ml 1 x 10ml -
Research areas
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Alternative names
- 5 mC
- 5 MethylCytosine
- 5mC
Images
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Functional Studies - Methylated DNA Quantification Kit (Colorimetric) (ab117128)Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.
