Mouse SFTPC ELISA Kit (ab252366)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 40 pg/ml
- Range: 117.19 pg/ml - 7500 pg/ml
- Sample type: Tissue Culture Media, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse SFTPC ELISA Kit
See all Prosurfactant Protein C kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% extract 24 4.8% Inter-assay Sample n Mean SD CV% extract 3 5% -
Sample type
Tissue Extracts, Tissue Culture Media -
Assay type
Sandwich (quantitative) -
Sensitivity
40 pg/ml -
Range
117.19 pg/ml - 7500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 105 99% - 110% Tissue Culture Media 104 99% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse SFTPC ELISA Kit (ab252366) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SFTPC protein in tissue culture media and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse SFTPC with 40 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse SFTPC Capture Antibody 1 x 600µl 10X Mouse SFTPC Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse SFTPC Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. -
Involvement in disease
Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. -
Sequence similarities
Contains 1 BRICHOS domain. -
Cellular localization
Secreted > extracellular space > surface film. - Information by UniProt
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Alternative names
- BRICD6
- BRICHOS domain containing 6
- PSP C
see all -
Database links
- Entrez Gene: 20389 Mouse
- SwissProt: P21841 Mouse
- Unigene: 24040 Mouse
Images
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Other - Mouse SFTPC ELISA Kit (ab252366)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse SFTPC standard curve in 1X Cell Extraction Buffer PTR.The SFTPC standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native SFTPC in mouse tissue supernatant and tissue samples.The concentrations of SFTPC were measured in duplicates, interpolated from the SFTPC standard curves and corrected for sample dilution. Undiluted samples are as follows: Mouse lung supernatant 50%, mouse lung extract 25 mg/mL. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SFTPC concentration was determined to be 5.31 ng/mL in lung supernatant and 3.99 ng/mL in lung extract.
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Sandwich ELISA - Mouse SFTPC ELISA Kit (ab252366)To learn more about the advantages of recombinant antibodies see here.
