Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMEM192 antibody [EPR14330] (ab185545)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14330] to TMEM192
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TMEM192 antibody [EPR14330]
    See all TMEM192 primary antibodies

  • Description

    Rabbit monoclonal [EPR14330] to TMEM192

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • U87-MG cell & Human fetal liver lysates, HepG2 & U87-MG cells,

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14330

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-TMEM192 antibody [EPR14330] (ab185545)
    Flow Cytometry - Anti-TMEM192 antibody [EPR14330] (ab185545)

    Flow cytometry analysis using ab185545 at 1/240 dilution on U87-MG cells.  Secondary ab: Goat anti rabbit IgG (FITC) at 1/250 dilution.  Fixative: 2% paraformaldehyde.  Isotype control: Rabbit monoclonal IgG.

  • Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody [EPR14330] (ab185545)
    Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody [EPR14330] (ab185545)

    Immunofluorescence analysis of HepG2 cells labeling TMEM192 with ab185545 at 1/100 dilution.  Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.  Fixative: 4% paraformaldehyde.  Counter stain: Dapi.

  • Western blot - Anti-TMEM192 antibody [EPR14330] (ab185545)
    Western blot - Anti-TMEM192 antibody [EPR14330] (ab185545)
    Anti-TMEM192 antibody [EPR14330] (ab185545) at 1/2000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 31 kDa



    Blocking buffer: 5% NFDM/TBST

  • Western blot - Anti-TMEM192 antibody [EPR14330] (ab185545)
    Western blot - Anti-TMEM192 antibody [EPR14330] (ab185545)
    Anti-TMEM192 antibody [EPR14330] (ab185545) at 1/10000 dilution + U87-MG cell lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 31 kDa



    Blocking buffer: 5% NFDM/TBST

  • Anti-TMEM192 antibody [EPR14330] (ab185545)
    Anti-TMEM192 antibody [EPR14330] (ab185545)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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