Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Wednesday March 10, 2021

Anti-TMEM192 antibody [EPR14330] (ab185545)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14330] to TMEM192
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-TMEM192 antibody [EPR14330]
See all TMEM192 primary antibodies
Description

Rabbit monoclonal [EPR14330] to TMEM192
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- U87-MG cell & Human fetal liver lysates, HepG2 & U87-MG cells,
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14330
Isotype

IgG
Research areas

Flow Cytometry - Anti-TMEM192 antibody [EPR14330] (ab185545)

Flow cytometry analysis using ab185545 at 1/240 dilution on U87-MG cells. Secondary ab: Goat anti rabbit IgG (FITC) at 1/250 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody [EPR14330] (ab185545)

Immunofluorescence analysis of HepG2 cells labeling TMEM192 with ab185545 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
Western blot - Anti-TMEM192 antibody [EPR14330] (ab185545)

Anti-TMEM192 antibody [EPR14330] (ab185545) at 1/2000 dilution + Human fetal liver lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 31 kDa

Blocking buffer: 5% NFDM/TBST
Western blot - Anti-TMEM192 antibody [EPR14330] (ab185545)

Anti-TMEM192 antibody [EPR14330] (ab185545) at 1/10000 dilution + U87-MG cell lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 31 kDa

Blocking buffer: 5% NFDM/TBST
Anti-TMEM192 antibody [EPR14330] (ab185545)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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