Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Kaiso antibody [6F / 6F8] (ab12723)

Key features and details

  • Mouse monoclonal [6F / 6F8] to Kaiso
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Kaiso antibody [6F / 6F8]
    See all Kaiso primary antibodies

  • Description

    Mouse monoclonal [6F / 6F8] to Kaiso

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Fusion protein corresponding to Human Kaiso.

  • Positive control

    • CSML0 cells (IP then Western). In Flow Cytometry, this antibody gave a positive signal in HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    We can conjugate this antibody to FITC for you (please see ab150256 for details).

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
    Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
    Immunofluorescence using clone 6F / 6F8 on C2C12 cells.
  • Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
    Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)

    Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.

    Kaiso was IP'd from CSML0 cells using clone 6F / 6F8 and western blotted using clone 6F / 6F8.
  • Flow Cytometry - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
    Flow Cytometry - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
    Overlay histogram showing HeLa cells stained with ab12723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
    Immunocytochemistry/ Immunofluorescence - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723) Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.
    Immunofluroesence analysis of Human ARPE-19 cells, staining Kaiso using ab12723.
  • Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)
    Western blot - Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723) Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9.; Fig 4.; doi:10.1371/journal.pone.0036864; May 9, 2012, PLoS ONE 7(5): e36864.
    Representative western blot detecting Kaiso in ARPE-19 cells using ab12723.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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