Sphingomyelin Assay Kit (Fluorometric) (ab138877)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Whole Blood
- Sensitivity: 1 µM
Overview
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Product name
Sphingomyelin Assay Kit (Fluorometric)
See all Sphingomyelin kits -
Detection method
Fluorescent -
Sample type
Cell culture extracts, Whole Blood -
Assay type
Quantitative -
Sensitivity
1 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Sphingomyelin Assay Kit (Fluorometric) (ab138877) provides the most sensitive method for detecting neutral SM activity or screening SM inhibitors. The kit uses AbRed Indicator as a fluorogenic probe to indirectly quantify the phosphocholine produced from the hydrolysis of sphingomyelin (SM) by sphingomyelinase (SMase). It can be used for measuring the amount of SM in blood, cell extracts or other solutions. The fluorescence intensity of AbRed Indicator is proportional to the formation of phosphocholine, therefore to the amount of SM. AbRed Indicator enables the assay readable by either a fluorescence reader or an absorbance reader.
The kit is an optimized “mix and read” assay that can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.
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Notes
Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue. It plays an important role in signal transduction. Sphingomyelin accumulates abnormally in Niemann-Pick disease and Abetalipoproteinemia.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AbRed Indicator 1 vial Assay Buffer 1 x 5ml DMSO 1 x 200µl Enzyme Mix 1 vial SMase Reaction Buffer 1 x 20ml 50mM Sphingomyelin standard 1 x 20µl Sphingomyelinase 1 vial -
Research areas
Images
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Sample Standard Curve for SphingomyelinSphingomyelin dose response was measured on a solid black 96-well plate with ab138877 using a fluorescence microplate reader. As low as 1 µM sphingomyelin can be detected with 60 minutes incubation (n=3).
