Anti-p27 KIP 1 antibody (ab137736)
Key features and details
- Rabbit polyclonal to p27 KIP 1
- Suitable for: WB, IHC-P, ICC/IF, IP, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-p27 KIP 1 antibody
See all p27 KIP 1 primary antibodies -
Description
Rabbit polyclonal to p27 KIP 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IP, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cat, Dog
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Immunogen
Recombinant fragment corresponding to Human p27 KIP 1 aa 55-198.
Database link: P46527 -
Positive control
- WB: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, MCF-7 and MDA-MB-231 whole cell lysates; IHC: Human ulcerative colitis tissue. IGC-Fr: Mouse retina. IP: MCF-7 whole cell extract. ICC/ IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p27 KIP 1 antibody (ab137736)All lanes : Anti-p27 KIP 1 antibody (ab137736) at 1/500 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : p27 KIP 1 knockout HeLa whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 22 kDa12% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody (ab137736)Immunofluorescence staining of paraformaldehyde-fixed (4% paraformaldehyde, 15 minutes at RT) HeLa cells stained for p27 KIP1 with ab137736 at 1:500 dilution. Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
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Western blot - Anti-p27 KIP 1 antibody (ab137736)All lanes : Anti-p27 KIP 1 antibody (ab137736) at 1/1000 dilution
Lane 1 : Neuro 2A whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH-3T3 whole cell extract
Lane 4 : Raw264.7 whole cell extract
Lane 5 : C2C12 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa12% SDS-PAGE
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Immunohistochemistry (Frozen sections) - Anti-p27 KIP 1 antibody (ab137736)Frozen sections of adult mouse retina tissue stained for p27 KIP1 using ab137736 at 1:250 dilution in immunohistochemical analysis. Red: protein kinase C alpha staining. Blue: Fluoroshield with DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody (ab137736)Immunohistochemical analysis of paraffin embedded human ulcerative colitis tissue labelling p27 KIP 1 with ab137736 antibody.
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Immunoprecipitation - Anti-p27 KIP 1 antibody (ab137736)5 μg of ab137736 immunoprecipitating p27 KIP1 in MCF-7 whole cell extract.
Lane 1 (input): Control IgG + MCF-7 whole cell lysate
Lane 2 (+): ab137736 + MCF-7 whole cell lysate.
Western blot analysis was performed using ab137736.
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Western blot - Anti-p27 KIP 1 antibody (ab137736)All lanes : Anti-p27 KIP 1 antibody (ab137736) at 1/1000 dilution
Lane 1 : MCF-7 cell extract
Lane 2 : MDA-MB-231 cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 22 kDa12% SDS-PAGE
