RhoC Activation Assay Kit (ab211166)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
RhoC Activation Assay Kit
See all RHOC kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
RhoC Activation Assay Kit (ab211166) uses Rhotekin RBD Agarose beads to selectively isolate and pull-down the active form of Rho from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rho is detected by western blot analysis using an anti-RhoC specific monoclonal antibody, which detects the human, mouse and rat samples.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
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Notes
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
RhoC is a 21 kDa protein that regulates a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, RhoC cycles between an inactive GDP-bound form and an active GTP-bound form: in its active (GTP-bound) state, RhoC binds specifically to the Rho-binding domain (RBD) of Rhotekin to control downstream signaling cascades.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 20 tests ab211178 - Rhotekin RBD Agarose Beads 1 x 200µl 100X GTPyS 1 x 20µl 100X GDP 1 x 20µl 5X Assay/Lysis Buffer 4 x 2ml Anti-rhoC mouse monoclonal antibody 1 x 10µl RhoC immunoblot Positive Control 1 x 100µl -
Research areas
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Function
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Aplysia RAS-related homolog 9
- ARH 9
- ARH9
see all -
Database links
- Entrez Gene: 389 Human
- Entrez Gene: 11853 Mouse
- Entrez Gene: 295342 Rat
- Omim: 165380 Human
- SwissProt: P08134 Human
- SwissProt: Q62159 Mouse
- Unigene: 502659 Human
- Unigene: 658289 Human
see all
Images
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RhoC Activation Assay Kit (ab211166)
RhoC positive control (recombinant human RhoC from E. coli, partially purified) detected by immunoblot.
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RhoC Activation Assay Kit (ab211166)Dot blot that demonstrates specificity of anti-RhoC mouse antibody.