Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18133] to RhoA + RhoC - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free
    See all RhoA + RhoC primary antibodies

  • Description

    Rabbit monoclonal [EPR18133] to RhoA + RhoC - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse kidney tissue.

  • General notes

    Ab238943 is the carrier-free version of ab187026. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238943 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling RhoA + RhoC with ab187026 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on cancer cells of transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187026).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling RhoA + RhoC with ab187026 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on epithelial cells of rat stomach is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187026).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)
    Immunocytochemistry/ Immunofluorescence - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling RhoA + RhoC with ab187026 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on NIH 3T3 cells is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab187026 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187026).

  • Flow Cytometry - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)
    Flow Cytometry - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling RhoA + RhoC with ab187026 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187026).

  • Immunoprecipitation - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)
    Immunoprecipitation - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)

    Immunoprecipitation of RhoA + RhoC from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate achieved using ab187026 at 1/50 dilution.
    Lane 1: Input: 10µg of NIH/3T3 whole cell lysate.
    Lane 2: NIH/3T3 whole cell lysate following IP with ab187026.
    Lane 3: Negative control: IP using Rabbit monoclonal IgG (ab172730) instead of ab187026 in NIH/3T3 whole cell lysates.
    Western blot was performed using ab187026 at 1/1000 dilution.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 30 second exposure.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187026).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling RhoA + RhoC with ab187026 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on kidney tubules of mouse kidney is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187026).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)
    Anti-RhoA + RhoC antibody [EPR18133] - BSA and Azide free (ab238943)

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