Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2731] to Tau (phospho S396) - BSA and Azide free
  • Suitable for: WB, IP, Dot blot, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR2731] to Tau (phospho S396) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Dot blot, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY5Y treated with alkaline phosphatase, Human and Mouse brain tissue lysate; IHC-P: human glioblastoma, human Alzheimer hippocampus, human, mouse and rat colon; IP- Human brain lysate; IHC-Fr: Mouse and Rat cerebrum tissue, Hu Alzheimer brain.

  • General notes

    ab156623 is the carrier-free version of ab109390.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)

    Immunohistochemistry (Frozen sections) analysis of rat cerebrum tissue sections labeling Tau with Purified ab109390 at 1/100 (1.0 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109390).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)

    Immunohistochemical staining of paraffin embedded human glioblastoma with purified ab109390 at a dilution of 1/4000. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109390).

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling Tau with Purified ab109390 at 1/100 (1.0 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109390).

  • Immunoprecipitation - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)
    Immunoprecipitation - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)

    ab109390 at 1/20 immunoprecipitating Tau (phospho S396) in Human brain lysate.

    Lane 1 (input): Human brain lysate (10µg)

    Lane 2 (+): ab109390 + Human brain lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109390 in Human brain lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Diluting / Blocking buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109390).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623) This image is courtesy of an Abreview submitted by Carl Hobbs.

    IHC image of Tau (phospho S396) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with unpurified ab109390 at 1/1000 dilution for 2 hoursat 21ºC. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109390).

  • Dot Blot - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)
    Dot Blot - Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)

    Dot blot analysis of Tau (phospho S396) phospho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labeling Tau (phospho S396) with ab109390 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109390).

  • Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)
    Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free (ab156623)

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