Anti-Tau antibody [5B10] (ab278070)
![Anti-Tau antibody [5B10] (ab278070)](https://www.abcam.com/ps/products/278/ab278070/Images/ab278070-445722-AntiTau-antibody-5B10-ICC-Embryonic-rat-E20-cortical-neuronglial-cells.JPG "Anti-Tau antibody [5B10] (ab278070)")
Key features and details
- Mouse monoclonal [5B10] to Tau
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-Tau antibody [5B10]
See all Tau primary antibodies -
Description
Mouse monoclonal [5B10] to Tau -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human Tau aa 1-758.
Database link: P10636 -
Epitope
HVPGGGNKKIETHKLTFREN -
Positive control
- WB: Rat brain and Spinal cord, Mouse brain and Spinal cord. ICC: Embryonic rat E20 cortical neuron-glial cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
5B10 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-Tau antibody [5B10] (ab278070)
Cortical neuron-glial cell culture from E20 rat stained for MAP-τ (green) using ab278070 at 1/2000 dilution in immunocytochemistry. A chicken polyclonal antibody to GFAP (red) was used at 1/5000 dilution. The blue is DAPI staining of nuclear DNA.
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![Western blot - Anti-Tau antibody [5B10] (ab278070)](https://www.abcam.com/ps/products/278/ab278070/Images/ab278070-445719-AntiTau-antibody-5B10-WB-Rat-brain-and-Spinal-cord-Mouse-brain-and-Spinal-cord.JPG)
Western blot - Anti-Tau antibody [5B10] (ab278070)Lanes 2-5 : Anti-Tau antibody [5B10] (ab278070) at 1/2000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : rat brain
Lane 3 : rat spinal cord
Lane 4 : mouse brain
Lane 5 : mouse spinal cord
Predicted band size: 78 kDaTau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the region of the blot from 48 kDa to 67 kDa with some higher molecular weight isoforms.