Anti-FOXP1 antibody [JC12] (ab32010)
![Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.com/ps/products/32/ab32010/Images/ab32010-274940-anti-foxp1-antibody-jc12-immunohistochemistry.jpg "Anti-FOXP1 antibody [JC12] (ab32010)")
Key features and details
- Mouse monoclonal [JC12] to FOXP1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-FOXP1 antibody [JC12]
See all FOXP1 primary antibodies -
Description
Mouse monoclonal [JC12] to FOXP1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Full length native protein (purified) corresponding to Mouse FOXP1.
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Positive control
- WB: BCBL1 human cell line or Mutul human cell line. IHC-P: Human Hodgkin's lymphoma and human colon tissues.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 6.97% L-Arginine, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
JC12 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)
IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.com/ps/products/32/ab32010/Images/ab32010-43628-anti-foxp1-antibody-jc12-western-blot.jpg)
Western blot - Anti-FOXP1 antibody [JC12] (ab32010)All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 10 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HEK293 Whole Cell Lysate Overexpressing FOXP1
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
Predicted band size: 75 kDa
Observed band size: 100,80 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.com/ps/products/32/ab32010/Images/ab32010-274942-anti-foxp1-antibody-jc12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human Hodgkin's lymphoma (CD30+)*, showing positive staining in the mantle zone and negative staining in the Hodgkin's cells and germinal centre. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.com/ps/products/32/ab32010/Images/ab32010-43629-anti-foxp1-antibody-jc12-western-blot.jpg)
Western blot - Anti-FOXP1 antibody [JC12] (ab32010)All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 1/500 dilution
Lane 1 : BCBL1 human cell line
Lane 2 : Mutul human cell line
Predicted band size: 75 kDa
Observed band size: 60,65,80 kDa why is the actual band size different from the predicted?
This antibody recognizes a long form and two short forms of human FOXP1 as described in Blood. 2008. Mar 1; 111(5): 2816-24.
Image kindly provided by H. Hu.
