Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-FOXP1 antibody [EPR4113] (ab134055)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4113] to FOXP1
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-FOXP1 antibody [EPR4113]
See all FOXP1 primary antibodies
Description

Rabbit monoclonal [EPR4113] to FOXP1
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human FOXP1 aa 350-450. The exact sequence is proprietary.
Positive control
- Daudi, MCF7, T47D, 2.4G2, RAW264.7 and Jurkat whole cell lysate (ab7899); mouse lung tissue lysates, Human tonsil tissue, Human diffuse large B-cell lymphoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4113
Isotype

IgG
Research areas

Western blot - Anti-FOXP1 antibody [EPR4113] (ab134055)

Anti-FOXP1 antibody [EPR4113] (ab134055) at 1/5000 dilution (Purified) + 2.4G2 (Rat B cell lymphoma B lymphocyte) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 75 kDa
Observed band size: 50,70,95 kDa
why is the actual band size different from the predicted?

The multiple bands are consistent with what have been described in the literature PMID: 12692134.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human diffuse large B-cell lymphoma tissue sections labeling FOXP1 with purified ab134055 at 1/1000 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-FOXP1 antibody [EPR4113] (ab134055)

All lanes : Anti-FOXP1 antibody [EPR4113] (ab134055) at 1/1000 dilution (Purified)

Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 3 : Mouse lung cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 75 kDa
Observed band size: 50,70,95 kDa why is the actual band size different from the predicted?

The multiple bands are consistent with what have been described in the literature PMID: 12692134.
Immunocytochemistry/ Immunofluorescence - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling FOXP1 with purified ab134055 at 1/500 dilution (0.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab134055 (unpurified) showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab134055 (unpurified) showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling FOXP1 with ab134055 (unpurified) at 1/250 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab134055 (unpurified) showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab134055 (unpurified) showing -ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab134055 (unpurified) showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Flow Cytometry - Anti-FOXP1 antibody [EPR4113] (ab134055)

Flow Cytometry analysis of Daudi (human Burkitt's lymphoma ) cells labeling FOXP1 with purified ab134055 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab134055 (unpurified) showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (ab134055)

Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab134055 (unpurified) showing -ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-FOXP1 antibody [EPR4113] (ab134055)

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