Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACTN3 antibody [EP2531Y] (ab68204)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2531Y] to ACTN3
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ACTN3 antibody [EP2531Y]
    See all ACTN3 primary antibodies

  • Description

    Rabbit monoclonal [EP2531Y] to ACTN3

  • Host species

    Rabbit

  • Specificity

    The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ACTN3 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human skeletal muscle, Mouse skeletal muscle, and Rat skeletal muscle lysates. IHC-P: human skeletal muscle tissue. Flow Cyt: L6 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    All lanes : Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/20000 dilution (Purified)

    Lane 1 : Mouse skeletal muscle lysates
    Lane 2 : Rat skeletal muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 103 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue sections labeling ACTN3 with purified ab68204 at 1/250 dilution (0.97 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Flow Cytometry - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Flow Cytometry analysis of L6 (Rat skeletal muscle myoblast) cells labeling ACTN3 with purified ab68204 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    All lanes : Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/5000 dilution (Purified)

    Lane 1 : Human skeletal muscle lysates at 15 µg


    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 103 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/10000 dilution ((unpurified)) + human skeletal muscle lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 103 kDa
    Observed band size: 103 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)

    Immunohistochemical analysis of paraffin-embedded human muscle tissue using unpurified ab68204 at 1/100-1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Anti-ACTN3 antibody [EP2531Y] (ab68204)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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