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Anti-FOXP1 antibody [JC12] - BSA and Azide free (ab264529)

Key features and details

  • Mouse monoclonal [JC12] to FOXP1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-FOXP1 antibody [JC12] - BSA and Azide free
    See all FOXP1 primary antibodies

  • Description

    Mouse monoclonal [JC12] to FOXP1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Full length native protein (purified) corresponding to Mouse FOXP1.

  • Positive control

    • IHC-P: Human Hodgkin's lymphoma and human colon tissues.

  • General notes

    ab264529 is the carrier-free version of ab32010.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] - BSA and Azide free (ab264529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] - BSA and Azide free (ab264529)

    IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32010).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] - BSA and Azide free (ab264529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] - BSA and Azide free (ab264529)

    IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human Hodgkin's lymphoma (CD30+)*, showing positive staining in the mantle zone and negative staining in the Hodgkin's cells and germinal centre. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 

    The inset negative control image is taken from an identical assay without primary antibody.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32010).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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