Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box

Anti-E2F1 antibody (ab245307)

Anti-E2F1 antibody (ab245307)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to E2F1
  • Suitable for: IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Product image
Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
Product image
Anti-FOXE1 antibody (ab236661)
Human FOXO3 (FOXO3A) knockout Hep G2 cell line (ab277869)
Product image
Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594)

Overview

  • Product name

    Anti-E2F1 antibody
    See all E2F1 primary antibodies
  • Description

    Rabbit polyclonal to E2F1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human E2F1 aa 300-350. The exact sequence is proprietary. (NP_005216.1).
    Database link: Q01094

  • Positive control

    • IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245307 was affinity purified using an epitope specific to E2F1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family

Images

  • Immunoprecipitation - Anti-E2F1 antibody (ab245307)
    Immunoprecipitation - Anti-E2F1 antibody (ab245307)

    E2F1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245307 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative rabbit anti-E2F1 antibody, at 1 µg/ml.

    Lane 1: ab245307 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody (ab245307)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody (ab245307)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for E2F1 using ab245307 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Endo G antibody [EP1665Y] (ab76122)

  •  
  • Product image

    Anti-Ubiquitin antibody (ab7780)

  •  
  • Product image

    Anti-Keratin 12/K12 antibody [EPR17883] (ab185626)

  •  
  • Product image

    Anti-AFM antibody [EPR19383] (ab201034)

  •  
  • Product image

    Anti-TRRAP antibody [EPR14952] (ab183517)

  •  
  • Product image

    Anti-ADX antibody [EPR4630] (ab109312)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.