Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXF1 antibody [EPR7971] (ab168383)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7971] to FOXF1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-FOXF1 antibody [EPR7971]
    See all FOXF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7971] to FOXF1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human FOXF1. The exact sequence is proprietary.
    Database link: Q12946

  • Positive control

    • Flow Cyt: HeLa cells; ICC/IF: HeLa cells; WB: HeLa, and Human lung lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-FOXF1 antibody [EPR7971] (ab168383)
    Western blot - Anti-FOXF1 antibody [EPR7971] (ab168383)
    All lanes : Anti-FOXF1 antibody [EPR7971] (ab168383) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human lung lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    This antibody detects high background

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] (ab168383)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] (ab168383)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1:250 dilution (2.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-FOXF1 antibody [EPR7971] (ab168383)
    Flow Cytometry - Anti-FOXF1 antibody [EPR7971] (ab168383)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1/70 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Anti-FOXF1 antibody [EPR7971] (ab168383)
    Anti-FOXF1 antibody [EPR7971] (ab168383)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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