Anti-FOXC2 antibody (ab5060)
Key features and details
- Goat polyclonal to FOXC2
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-FOXC2 antibody
See all FOXC2 primary antibodies -
Description
Goat polyclonal to FOXC2 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment
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Immunogen
Synthetic peptide:
RHAAPYSYDCTKY
, corresponding to C terminal amino acids 489-501 of Human FOXC2. -
Positive control
- WB: HEK-293 nuclear lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FOXC2 antibody (ab5060)Lane 1 : Anti-FOXC2 antibody (ab5060) at 2 µg/ml
Lane 2 : Anti-FOXC2 antibody (ab5060) at 1 µg/ml
Lane 1 : nuclear HEK293 lysate in RIPA buffer
Lane 2 : human pancreas whole cell lysate (negative control) in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 60-70 kDa why is the actual band size different from the predicted?Primary incubation: 1 hour at room temperature.
Negative Control: Human Pancreas lysate.
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Western blot - Anti-FOXC2 antibody (ab5060) Image courtesy of a review by Michal Bell submitted 19 August 2004FoxC2 is highly expressed in COS7 and 293 cells transiently transfected with pcDNA-FOXC2. This figure shows a western blot incubated with the ab5060 antibody after running lysates from COS7 and 293 cells transiently expressing FoxC2 (lane 1 and 3 respectively). Lane 2 shows a negative control of 293 cells transfected with an empty vector.
