Anti-FOXO4/AFX (phospho S197) antibody (ab47278)
Key features and details
- Rabbit polyclonal to FOXO4/AFX (phospho S197)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXO4/AFX (phospho S197) antibody
See all FOXO4/AFX primary antibodies -
Description
Rabbit polyclonal to FOXO4/AFX (phospho S197) -
Host species
Rabbit -
Specificity
This (phospho-Ser197) antibody detects endogenous levels of FOXO4/AFX only when phosphorylated at serine 197.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human FOXO4/AFX. derived from human FOXO4/AFX around the phosphorylation site of serine 197 (A-A-SP-M-D)
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General notes
Previously labelled as FOXO4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FOXO4/AFX (phospho S197) antibody (ab47278)All lanes : Anti-FOXO4/AFX (phospho S197) antibody (ab47278) at 1/500 dilution
Lane 1 : Extracts from 293 cells.
Lane 2 : Extracts from 293 cells. Immunizing peptide 1ug/mL
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Peptide - + -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX (phospho S197) antibody (ab47278)This image shows human breast carcinoma stained with ab47781 at 1/50 dilution. Right hand image:tissue was treated with immunogenic peptide, left hand image: untreated.
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Immunocytochemistry/ Immunofluorescence - Anti-FOXO4/AFX (phospho S197) antibody (ab47278)ICC/IF image of ab47278 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47278, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
