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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box

Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876)

Price and availability

251 280 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2314(2)] to FOXO4/AFX (phospho T32)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)]
    See all FOXO4/AFX primary antibodies
  • Description

    Rabbit monoclonal [EPR2314(2)] to FOXO4/AFX (phospho T32)
  • Host species

    Rabbit
  • Specificity

    Only detects FOXO4 / AFX phosphorylated at Threonine 32.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human FOXO4/AFX.

  • Positive control

    • 293T transfected with HA-FOXO4/AFX
  • General notes

    This antibody was developed as part of a collaboration with Tobais Dansen from the University of Utrecht in the Netherlands.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as FOXO4. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Dissociation constant (KD)

    KD = 2.51 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2314(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXO

Images

  • Western blot - Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876)
    Western blot - Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876)
    All lanes : Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876) at 1/1000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : 293T transfected with HA-FOXO4/AFX cell lysates
    Lane 3 : 293T transfected with HA-FOXO4.A3 (T32, S197 and S262 mutated to A) cell lysates

    Predicted band size: 53 kDa

  • OI-RD Scanning - Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876)
    OI-RD Scanning - Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876)
    Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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