Anti-E2F1 antibody [KH95] (ab4070)
![Anti-E2F1 antibody [KH95] (ab4070)](https://www.abcam.com/ps/products/4/ab4070/Images/ab4070-451727-anti-e2f1-antibody-kh95-western-blot-wildtype-hek293-e2f1-knockout-hek293-wildtype-hek293-e2f1-knockout-hek29.jpg "Anti-E2F1 antibody [KH95] (ab4070)")
Key features and details
- Mouse monoclonal [KH95] to E2F1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-E2F1 antibody [KH95]
See all E2F1 primary antibodies -
Description
Mouse monoclonal [KH95] to E2F1 -
Host species
Mouse -
Specificity
Protein of 60 kDa. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant full length protein (Human).
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Positive control
- WB: HepG2, HEK-293, HeLa and U-2 OS cell lysates. IHC-P: Human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: Ascites, 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
KH95 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-E2F1 antibody [KH95] (ab4070)All lanes : Anti-E2F1 antibody [KH95] (ab4070) at 1/500 dilution
Lane 1 : Wild-type HEK-293 cell lysate at 40 µg
Lane 2 : E2F1 knockout HEK-293 cell lysate at 40 µg
Lane 3 : HepG2 cell lysate at 20 µg
Lane 4 : U-2 OS cell lysate at 20 µg
Lane 5 : HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Lanes 1 - 5: Merged signal (red and green). Green - ab4070 observed at 55 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.
ab4070 was shown to react with E2F1 in wild-type HEK-293 cells in Western blot with loss of signal observed in E2F1 knockout sample. Wild-type HEK-293 and E2F1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab4070 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (ab4070)](https://www.abcam.com/ps/products/4/ab4070/Images/ab4070_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (ab4070)ab4070 staining human tonsil by IHC-P.
