Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-E2F1 antibody [KH95] (ab4070)

Key features and details

  • Mouse monoclonal [KH95] to E2F1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-E2F1 antibody [KH95]
    See all E2F1 primary antibodies

  • Description

    Mouse monoclonal [KH95] to E2F1

  • Host species

    Mouse

  • Specificity

    Protein of 60 kDa.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein (Human).

  • Positive control

    • WB: HepG2, HEK-293, HeLa and U-2 OS cell lysates. IHC-P: Human tonsil tissue.

Properties

Images

  • Western blot - Anti-E2F1 antibody [KH95] (ab4070)
    Western blot - Anti-E2F1 antibody [KH95] (ab4070)
    All lanes : Anti-E2F1 antibody [KH95] (ab4070) at 1/500 dilution

    Lane 1 : Wild-type HEK-293 cell lysate at 40 µg
    Lane 2 : E2F1 knockout HEK-293 cell lysate at 40 µg
    Lane 3 : HepG2 cell lysate at 20 µg
    Lane 4 : U-2 OS cell lysate at 20 µg
    Lane 5 : HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 5: Merged signal (red and green). Green - ab4070 observed at 55 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

    ab4070 was shown to react with E2F1 in wild-type HEK-293 cells in Western blot with loss of signal observed in E2F1 knockout sample. Wild-type HEK-293 and E2F1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab4070 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (ab4070)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (ab4070)
    ab4070 staining human tonsil by IHC-P.

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