Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2150(3)] to FOXO4/AFX
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-FOXO4/AFX antibody [EPR2150(3)]
    See all FOXO4/AFX primary antibodies

  • Description

    Rabbit monoclonal [EPR2150(3)] to FOXO4/AFX

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human FOXO4/AFX. The exact sequence is proprietary.

  • Positive control

    • HA-FOXO4/AFX transfected 293T cell lysate; HA-FOXO4.A3 (T32, S197 and S262 mutated to A) transfected 293T cell lysate; Human placenta tissue.

  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the University of Utrecht, Netherlands and the lab of Tobias Dansen.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as FOXO4. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
    Western blot - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
    All lanes : Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757) at 1/10000 dilution

    Lane 1 : HA-FOXO4/AFX transfected 293T cell lysate
    Lane 2 : HA-FOXO4.A3 (T32, S197 and S262 mutated to A) transfected 293T cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)

    ab126757, at 1/100 dilution, staining FOXO4/AFX in paraffin-embedded Human placenta tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
    OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
    Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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