Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
![Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)](https://www.abcam.com/ps/products/126/ab126757/Images/ab126757-140782-anti-foxo4-afx-antibody-epr21503-western-blot.jpg "Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2150(3)] to FOXO4/AFX
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-FOXO4/AFX antibody [EPR2150(3)]
See all FOXO4/AFX primary antibodies -
Description
Rabbit monoclonal [EPR2150(3)] to FOXO4/AFX -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human FOXO4/AFX. The exact sequence is proprietary.
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Positive control
- HA-FOXO4/AFX transfected 293T cell lysate; HA-FOXO4.A3 (T32, S197 and S262 mutated to A) transfected 293T cell lysate; Human placenta tissue.
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General notes
This antibody was developed as part of a collaboration between Epitomics, the University of Utrecht, Netherlands and the lab of Tobias Dansen.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as FOXO4.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.81 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2150(3) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)All lanes : Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757) at 1/10000 dilution
Lane 1 : HA-FOXO4/AFX transfected 293T cell lysate
Lane 2 : HA-FOXO4.A3 (T32, S197 and S262 mutated to A) transfected 293T cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 53 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)](https://www.abcam.com/ps/products/126/ab126757/Images/ab126757-140781-anti-foxo4-afx-antibody-epr21503-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)ab126757, at 1/100 dilution, staining FOXO4/AFX in paraffin-embedded Human placenta tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)](https://www.abcam.com/ps/products/126/ab126757/Images/ab126757-140780-anti-foxo4-afx-antibody-epr21503.jpg)
OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757) -
![Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)](https://www.abcam.com/ps/products/126/ab126757/Images/ab126757-4-benefits-of-recombinant-antibodies.png)
Anti-FOXO4/AFX antibody [EPR2150(3)] (ab126757)
