Anti-FOXO4/AFX (phospho S193) antibody [EPR2150(N)] (ab174849)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2150(N)] to FOXO4/AFX (phospho S193)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-FOXO4/AFX (phospho S193) antibody [EPR2150(N)]
See all FOXO4/AFX primary antibodies -
Description
Rabbit monoclonal [EPR2150(N)] to FOXO4/AFX (phospho S193) -
Host species
Rabbit -
Specificity
ab174849 only detects FOXO4 / AFX phosphorylated at Serine 193. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
within Human FOXO4/AFX aa 150-250 (phospho S193). The exact sequence is proprietary.
Database link: P98177 -
Positive control
- FOXO4/AFX transfected 293Tcell lysate.
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General notes
This antibody was developed as part of a collaboration between Epitomics, University of Utrecht in the Netherlands, and the lab of Tobias Dansen.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as FOXO4.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2150(N) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FOXO4/AFX (phospho S193) antibody [EPR2150(N)] (ab174849) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : 293T cell lysate, transfected with FOXO4
Lane 3 : 293T cell lysate, transfected with FOXO4.A3 (T32A, S197(193)A, S262A)
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
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