Anti-FOXO4/AFX (phospho T451) antibody (ab79188)
Key features and details
- Rabbit polyclonal to FOXO4/AFX (phospho T451)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FOXO4/AFX (phospho T451) antibody
See all FOXO4/AFX primary antibodies -
Description
Rabbit polyclonal to FOXO4/AFX (phospho T451) -
Host species
Rabbit -
Specificity
ab79188 detects endogenous levels of FOXO4/AFX only when phosphorylated at threonine 451 (Human: Thr451; Mouse: Thr452).
-
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human FOXO4/AFX. derived from human FOXO4/AFX around the phosphorylation site of threonine 451 (L-G-TP-P-V)
-
Positive control
- Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins).
-
General notes
Previously labelled as FOXO4.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab79188 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-FOXO4/AFX (phospho T451) antibody (ab79188)All lanes : Anti-FOXO4/AFX (phospho T451) antibody (ab79188) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins)
Lane 2 : Extracts from HUVEC cells treated with EGF (200ng/ml, 5mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
