Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXO4/AFX antibody [EPR5442] (ab128908)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5442] to FOXO4/AFX
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-FOXO4/AFX antibody [EPR5442]
    See all FOXO4/AFX primary antibodies

  • Description

    Rabbit monoclonal [EPR5442] to FOXO4/AFX

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human FOXO4/AFX aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, fetal heart, fetal muscle, fetal lung, and HuT-78 lysates. Permeabilized 293T cells.

  • General notes

    This antibody was developed as part of a collaboration between the University of Utrecht in the Netherlands and the lab of Tobias Dansen.

    Previously labelled as FOXO4. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
    Western blot - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
    All lanes : Anti-FOXO4/AFX antibody [EPR5442] (ab128908) at 1/1000 dilution

    Lane 1 : 293T lysate
    Lane 2 : Fetal heart lysate
    Lane 3 : Fetal muscle lysate
    Lane 4 : Fetal lung lysate
    Lane 5 : HuT-78 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
    Flow Cytometry - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)

    Flow cytometric analysis of FOXO4/AFX in permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells using ab128908 at 1/100 dilution (Red) compared to a non-specific negative control antibody (green).

  • Immunoprecipitation - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
    Immunoprecipitation - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)

    Purified ab128908 at 1/100 dilution (2µg) immunoprecipitating FOXO4/AFX in 293T whole cell lysate.
    Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab128908 + 293T whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128908 in 293T whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 65 kDa

  • Western blot - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
    Western blot - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
    All lanes : Anti-FOXO4/AFX antibody [EPR5442] (ab128908) at 1/1000 dilution

    Lane 1 : 293T transfected with FOXO4/AFX
    Lane 2 : 293T cell lysate

    Secondary
    Lane 1 : Goat-anti-rabbit HRP at 1/2000 dilution
    Lane 2 : Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 63 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
    OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FOXO4/AFX antibody [EPR5442] (ab128908)
    Anti-FOXO4/AFX antibody [EPR5442] (ab128908)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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