Anti-FOXO4/AFX (phospho S193) antibody (ab2560)
Key features and details
- Rabbit polyclonal to FOXO4/AFX (phospho S193)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXO4/AFX (phospho S193) antibody
See all FOXO4/AFX primary antibodies -
Description
Rabbit polyclonal to FOXO4/AFX (phospho S193) -
Host species
Rabbit -
Specificity
This antibody recognizes the phosphorylated Ser193 of AFX. It does not react with non-phosphorylated AFX. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
This information is considered to be commercially sensitive.
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General notes
Previously labelled as FOXO4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FOXO4/AFX (phospho S193) antibody (ab2560)ICC/IF image of ab2560 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2560, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
