Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5442] to FOXO4/AFX - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free
    See all FOXO4/AFX primary antibodies

  • Description

    Rabbit monoclonal [EPR5442] to FOXO4/AFX - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HEK-293T cells. IP: 293T cell lysate

  • General notes

    ab232029 is the carrier-free version of ab128908 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232029 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as FOXO4.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)
    Immunoprecipitation - Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)

    Purified ab128908 at 1/100 dilution (2µg) immunoprecipitating FOXO4/AFX in 293T whole cell lysate.
    Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab128908 + 293T whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128908 in 293T whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 65 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128908).

  • Flow Cytometry - Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)
    Flow Cytometry - Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)

    Flow cytometric analysis of FOXO4/AFX in permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells using ab128908 at 1/100 dilution (Red) compared to a non-specific negative control antibody (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128908).

  • OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)
    OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128908).

  • Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)
    Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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