Antibodies, Proteins, Kits and Reagents for Life Science

Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5442] to FOXO4/AFX - BSA and Azide free
Suitable for: Flow Cyt, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free
See all FOXO4/AFX primary antibodies
Description

Rabbit monoclonal [EPR5442] to FOXO4/AFX - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, WB, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Flow Cyt: HEK-293T cells. IP: 293T cell lysate
General notes
ab232029 is the carrier-free version of ab128908 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab232029 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Previously labelled as FOXO4.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5442
Isotype

IgG
Research areas

Immunoprecipitation - Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)

Purified ab128908 at 1/100 dilution (2µg) immunoprecipitating FOXO4/AFX in 293T whole cell lysate.
Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab128908 + 293T whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128908 in 293T whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 65 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128908).
Flow Cytometry - Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)

Flow cytometric analysis of FOXO4/AFX in permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells using ab128908 at 1/100 dilution (Red) compared to a non-specific negative control antibody (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128908).
OI-RD Scanning - Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128908).
Anti-FOXO4/AFX antibody [EPR5442] - BSA and Azide free (ab232029)

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