Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17379] to FOXM1 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-FOXM1 antibody [EPR17379] - BSA and Azide free
    See all FOXM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17379] to FOXM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon and colon cancer tissues. ICC/IF: HeLa and HT-29 cells. WB: MEF cell lysate.

  • General notes

    ab232649 is the carrier-free version of ab207298. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232649 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)

    Immunohistochemical analysis of paraffin-embedded Human colon cancer  tissue labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on the Human colon cancer is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207298).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

     

    Confocal image showing nuclear staining on HeLa cell line. 

     

    The nuclear counterstain is DAPI (blue).

     

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR17379] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

     

    The negative controls are as follows:-

    -ve control 1: ab207298 at 1/250 dilution followed by ab150120  at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207298).

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

     

    Confocal image showing nuclear staining on HT-29 cell line.

     

    The nuclear counterstain is DAPI (blue).

     

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR17379] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

     

    The negative controls are as follows:-

    -ve control 1: ab207298 at 1/250 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207298).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FOXM1 with ab207298 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on epithelial cells of Human colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207298).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)
    Anti-FOXM1 antibody [EPR17379] - BSA and Azide free (ab232649)

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