Anti-FOXO3A antibody (ab17026)
Key features and details
- Goat polyclonal to FOXO3A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXO3A antibody
See all FOXO3A primary antibodies -
Description
Goat polyclonal to FOXO3A -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
GAKQASSQSWVPG
, corresponding to amino acids 661-673 of Human FOXO3A. -
Positive control
- Recombinant Human FOXO3A protein (ab114191) can be used as a positive control in WB. Human heart tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FOXO3A antibody (ab17026)All lanes : Anti-FOXO3A antibody (ab17026) at 0.3 µg/ml
Lane 1 : Human Heart tissue lysate
Lane 2 : Human Heart tissue lysate with immunising peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 72 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
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Western blot - Anti-FOXO3A antibody (ab17026) This image is courtesy of an anonymous AbreviewAll lanes : Anti-FOXO3A antibody (ab17026) at 1/1000 dilution
All lanes : Human hepatic epithelial-like cell line (AKN-1) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase conjugated-mouse anti-goat IgG monoclonal at 1/15000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 72 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible non-specific binding)
Exposure time: 5 minutesBlocked for 1 hour at 22°C.
Incubated with the primary antibody for 20 hours at 4°C.
