Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-E2F4 antibody [EPR8259] (ab150360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8259] to E2F4
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-E2F4 antibody [EPR8259]
    See all E2F4 primary antibodies

  • Description

    Rabbit monoclonal [EPR8259] to E2F4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human E2F4 aa 250-350. The exact sequence is proprietary.
    Database link: Q16254

  • Positive control

    • WB: HEK293T, K562, Raji, HeLa, and Jurkat (ab7899) cell lysates. IHC-P: Human kidney and lung adenocarcinoma tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-E2F4 antibody [EPR8259] (ab150360)
    Western blot - Anti-E2F4 antibody [EPR8259] (ab150360)
    All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : E2F4 knockout HEK293T cell lysate
    Lane 3 : K-562 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 44 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab150360 observed at 60 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab150360 Anti-E2F4 antibody [EPR8259] was shown to specifically react with E2F4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266119 (knockout cell lysate ab257932) was used. Wild-type and E2F4 knockout samples were subjected to SDS-PAGE. ab150360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-E2F4 antibody [EPR8259] (ab150360)
    Western blot - Anti-E2F4 antibody [EPR8259] (ab150360)
    All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 44 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

    Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

    Immunohistochemical analysis of paraffin embedded Human Thyroid gland papillary carcinoma tissue using ab150360 showing +ve staining.
    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

    Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab150360 showing +ve staining.
    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

    Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab150360 showing +ve staining.
    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150360 showing +ve staining.
    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-E2F4 antibody [EPR8259] (ab150360)
    Anti-E2F4 antibody [EPR8259] (ab150360)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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