Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8259] to E2F4
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-E2F4 antibody [EPR8259]
See all E2F4 primary antibodies
Description

Rabbit monoclonal [EPR8259] to E2F4
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human E2F4 aa 250-350. The exact sequence is proprietary.
Database link: Q16254
Positive control
- WB: HEK293T, K562, Raji, HeLa, and Jurkat (ab7899) cell lysates. IHC-P: Human kidney and lung adenocarcinoma tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8259
Isotype

IgG
Research areas

Western blot - Anti-E2F4 antibody [EPR8259] (ab150360)

All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : E2F4 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 44 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab150360 observed at 60 kDa. Red - loading control ab8245 observed at 36 kDa.

ab150360 Anti-E2F4 antibody [EPR8259] was shown to specifically react with E2F4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266119 (knockout cell lysate ab257932) was used. Wild-type and E2F4 knockout samples were subjected to SDS-PAGE. ab150360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-E2F4 antibody [EPR8259] (ab150360)

All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human Thyroid gland papillary carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody [EPR8259] (ab150360)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-E2F4 antibody [EPR8259] (ab150360)

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