Anti-E2F4 antibody (ab264276)
Key features and details
- Rabbit polyclonal to E2F4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-E2F4 antibody
See all E2F4 primary antibodies -
Description
Rabbit polyclonal to E2F4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human E2F4 aa 363-413. The exact sequence is proprietary. NP_001941.2
Database link: Q16254 -
Positive control
- IHC-P: Human prostate carcinoma tissue; IP: HeLa whole cell lysate; WB: HeLa and Hek-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E2F4 antibody (ab264276)All lanes : Anti-E2F4 antibody (ab264276) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Hek-293T whole cell lysate at 50 µg
Predicted band size: 44 kDaDetected with chemilimuniscence with exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F4 antibody (ab264276)Immunohistochemical analysis of FFPE human prostate carcinoma tissue section labelling E2F4 with ab264276 at 1/1000 dilution. Detection: DAB.
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Immunoprecipitation - Anti-E2F4 antibody (ab264276)E2F4 was immunoprecipitated from HEK-293T whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264276 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264276 at 1 μg/ml.
Lane 1: ab264276 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Exposure time:10 seconds.
