Indole Assay Kit (ab272536)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Other biological fluids
Overview
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Product name
Indole Assay Kit -
Detection method
Colorimetric -
Sample type
Other biological fluids -
Assay type
Quantitative -
Range
3 µM - 100 µM -
Product overview
Indole assay kit (272536) is based on a modified version of Ehlrich’s and Kovac’s reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample.
Fast and sensitive: Use of 100 μL sample. Linear detection range from 3 to 100 μM indole in 96-well plate assay.
Convenient: The procedure involves adding a single working reagent, and reading the absorbance immediately.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Reagent 1 x 12ml Standard (10 mM Indole) 1 x 100µl
Images
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Indole Assay Kit Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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E.coli Growth and Indole ConcentrationE.coli cells inoculated into 5 mM Tryptophan medium. Medium samples taken every two hours.
Data provided for demonstration purposes only.