Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] - BSA and Azide free (ab240127)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1951(2)] to FOXO3A (phospho S253) - BSA and Azide free
- Suitable for: Dot blot, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] - BSA and Azide free
See all FOXO3A primary antibodies -
Description
Rabbit monoclonal [EPR1951(2)] to FOXO3A (phospho S253) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab240127 is the carrier-free version of ab154786. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1951(2) -
Isotype
IgG -
Research areas
Images
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Dot Blot analysis of Lane 1: FOXO3A (pS253) phospho peptide and Lane 2: FOXO3A non-phospho peptide with ab154786 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer and concentration. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154786).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] - BSA and Azide free (ab240127)
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154786).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] - BSA and Azide free (ab240127)
Immunohistochemical analysis of paraffin-embedded Human normal pancreas tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154786).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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