Anti-FOXO3A antibody (ab70314)
Key features and details
- Rabbit polyclonal to FOXO3A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FOXO3A antibody
See all FOXO3A primary antibodies -
Description
Rabbit polyclonal to FOXO3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to a region between residues 250 and 300 of human FOXO3A. (Swiss-Prot O43524)
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Positive control
- Whole cell lysate from MCF7 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FOXO3A antibody (ab70314) at 0.4 µg/ml
Lane 1 : 293T cell Lysate
Lane 2 : Jurkat cell Lysate
Lane 3 : Mouse TCMK cell Lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 3 minutes
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Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg.
Lane 2: MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg
Lane 3:MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg
Western Blot carried out using ab70314 at 1ug/ml
Decction: ECL for 3 minutes.
All lanes : Anti-FOXO3A antibody (ab70314) at 1 µg/ml
Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg
Lane 2 : MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg
Lane 3 : MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg
Developed using the ECL technique.
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling FOXO3A with ab70314 at 1/500 (2µg/ml). Detection: DAB.