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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box

Anti-FOXO3A antibody (ab70314)

Anti-FOXO3A antibody (ab70314)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to FOXO3A
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FOXO3A antibody
    See all FOXO3A primary antibodies
  • Description

    Rabbit polyclonal to FOXO3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 250 and 300 of human FOXO3A. (Swiss-Prot O43524)

  • Positive control

    • Whole cell lysate from MCF7 cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXO

Images

  • Western blot - Anti-FOXO3A antibody (ab70314)
    Western blot - Anti-FOXO3A antibody (ab70314)
    All lanes : Anti-FOXO3A antibody (ab70314) at 0.4 µg/ml

    Lane 1 : 293T cell Lysate
    Lane 2 : Jurkat cell Lysate
    Lane 3 : Mouse TCMK cell Lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 71 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-FOXO3A antibody (ab70314)
    Immunoprecipitation - Anti-FOXO3A antibody (ab70314)

    Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg.

    Lane 2: MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg

    Lane 3:MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg

     

    Western Blot carried out using ab70314 at 1ug/ml

    Decction: ECL for 3 minutes.

     



    All lanes : Anti-FOXO3A antibody (ab70314) at 1 µg/ml

    Lane 1 : MCF7 Cell Lysate Immunoprecipitated with ab70314 at 6ug/mg
    Lane 2 : MCF7 Cell Lysate Immunoprecipitated with ab70315 6ug/mg
    Lane 3 : MCF7 Cell Lysate Immunoprecipitated with Control IgG at 6ug/mg

    Developed using the ECL technique.

    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody (ab70314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A antibody (ab70314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling FOXO3A with ab70314 at 1/500 (2µg/ml). Detection: DAB.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, WB

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    Applications: Dot, IHC-P, WB

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    Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)

    Applications: Dot, IHC-P, WB

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