Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1951(2)] to FOXO3A (phospho S253)
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-FOXO3A (phospho S253) antibody [EPR1951(2)]
See all FOXO3A primary antibodies -
Description
Rabbit monoclonal [EPR1951(2)] to FOXO3A (phospho S253) -
Host species
Rabbit -
Specificity
ab154786 only detects FOXO3A phosphorylated at Serine 253. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Phospho specific peptide corresponding to residues surrounding Serine 253 of Human FOXO3A (UniProt O43524).
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Positive control
- MCF-7 cell lysate treated with IGF; Human endometrial carcinoma tissue; Human pancreas tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1951(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786) at 1/5000 dilution
Lane 1 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with IGF-1. Whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
Immunohistochemical analysis of paraffin-embedded Human normal pancreas tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Dot Blot analysis of Lane 1: FOXO3A (pS253) phospho peptide and Lane 2: FOXO3A non-phospho peptide with ab154786 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer and concentration. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
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