Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-nkx6.1 antibody [EPR20405] (ab221549)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20405] to nkx6.1
  • Suitable for: IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-nkx6.1 antibody [EPR20405]
    See all nkx6.1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20405] to nkx6.1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal pancreas lysate. Beta-TC-6 whole cell lysate. IHC-P: Human pancreas and pancreatic neuroendocrine neoplasm tissues; Mouse pancreas tissue. IHC-Fr: Mouse pancreas tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling nkx6.1 with ab221549  at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on islet of mouse pancreas is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of human pancreas (PMID: 8702531). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

    Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine neoplasm tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly nuclear staining on tumor cells of human pancreatic neuroendocrine neoplasm (PMID: 25871618). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of mouse pancreas (PMID: 8702531). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Negative control: no staining on human colon (PMID: 25871618).

    Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Anti-nkx6.1 antibody [EPR20405] (ab221549) at 1/1000 dilution + Human fetal pancreas lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions.    

  • Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Anti-nkx6.1 antibody [EPR20405] (ab221549) at 1/1000 dilution + Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST

  • Anti-nkx6.1 antibody [EPR20405] (ab221549)
    Anti-nkx6.1 antibody [EPR20405] (ab221549)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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