Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Wednesday March 10, 2021

Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22491-81] to ITLN1 + ITLN2
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Rat, Human

Product name

Anti-ITLN1 + ITLN2 antibody [EPR22491-81]
Description

Rabbit monoclonal [EPR22491-81] to ITLN1 + ITLN2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged human ITLN2 and His-tagged human ITLN1 recombinant protein. Human small intestine tissue. Human fetal colon tissue. Rat and mouse colon tissue lysate. IHC-P: Human, rat and mouse colon tissue. IHC-Fr: Rat and mouse colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22491-81
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ITLN1 + ITLN2 with ab252926 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in goblet cells of human colon (PMID: 22768319) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

All lanes : Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926) at 1/1000 dilution

Lane 1 : Rat colon tissue lysate
Lane 2 : Rat small intestine tissue lysate
Lane 3 : Mouse colon tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35, 36 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

The actual band size higher than predicted mainly due to glycosylation. The molecular weight observed is consistent with what has been described in the literature (PMID: 11313366, PMID: 20628387). Lanes1-2 were developed using a higher sensitivity ECL substrate.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-2: 3 mins; Lane 3: 32 secs.
Immunohistochemistry (Frozen sections) - Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling ITLN1 + ITLN2 with ab252926 at 1/1250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on goblet cells of mouse colon (PMID: 22768319) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1,000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
Western blot - Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

All lanes : Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926) at 1/1000 dilution

Lane 1 : Human small intestine tissue lysate
Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 4 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : Human fetal colon tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1 & 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35, 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

The actual band size higher than predicted mainly due to glycosylation. The molecular weight observed is consistent with what has been described in the literature (PMID: 11313366, PMID: 20628387).

This blot was developed using a higher sensitivity ECL substrate.

Negative control: PC-3, A549, Caco-2 (PMID: 20628387).

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 3 mins; Lane 2-5: 15 secs.
Western blot - Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

All lanes : Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926) at 1/1000 dilution

Lane 1 : His-tagged human ITLN2 recombinant protein, 20 ng
Lane 2 : His-tagged human ITLN1 recombinant protein, 20 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35, 36 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Frozen sections) - Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling ITLN1 + ITLN2 with ab252926 at 1/1250 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on goblet cells of rat colon (PMID: 22768319) is observed. Counterstained with DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1,000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling ITLN1 + ITLN2 with ab252926 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in goblet cells of rat colon (PMID: 22768319) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling ITLN1 + ITLN2 with ab252926 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in goblet cells of mouse colon (PMID: 22768319) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-ITLN1 + ITLN2 antibody [EPR22491-81] (ab252926)

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