Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab58416)
Key features and details
- Rabbit polyclonal to E3 SUMO-protein ligase PIAS4/PIASy
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody
See all E3 SUMO-protein ligase PIAS4/PIASy primary antibodies -
Description
Rabbit polyclonal to E3 SUMO-protein ligase PIAS4/PIASy -
Host species
Rabbit -
Specificity
Detects endogenous levels of PAIAS gamma total protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human E3 SUMO-protein ligase PIAS4/PIASy.
-
Positive control
- Jurkat cell extract.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab58416)All lanes : Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab58416) at 1/500 dilution
Lane 1 : Jurkat cell extract without immunizing peptide
Lane 2 : Jurkat cell extract with immunizing peptide
Predicted band size: 57 kDa
Observed band size: 57 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab58416)IHC image of ab58416 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58416, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
