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Anti-UBE2E3 antibody [OTI4B4] (ab128098)

Key features and details

  • Mouse monoclonal [OTI4B4] to UBE2E3
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-UBE2E3 antibody [OTI4B4]
    See all UBE2E3 primary antibodies

  • Description

    Mouse monoclonal [OTI4B4] to UBE2E3

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human UBE2E3 aa 1-207. (NP_006348) produced in E.coli.
    Sequence:

    MSSDRQRSDDESPSTSSGSSDADQRDPAAPEPEEQEERKPSATQQKKNTK LSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGP PGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILK DNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQ WTKRYAT


    Database link: Q969T4
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HepG2 extracts; HEK-293T cells transiently transfected with the UBE2E3 cDNA. IHC-P: Human endometrium adenocarcinoma tissue. ICC/IF: HepG2 cells. Flow Cyt: HeLa and Jurkat cells; HEK-293T cells transfected with UBE2E3 overexpressing plasmid.

  • General notes

    Clone OTI4B4 (formerly 4B4).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
    Western blot - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
    Anti-UBE2E3 antibody [OTI4B4] (ab128098) at 1/200 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) extracts at 10 µg

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2E3 antibody [OTI4B4] (ab128098)

    Paraffin-embedded human endometrium adenocarcinoma tissue stained for UBE2E3 with ab128098 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
    Immunocytochemistry/ Immunofluorescence - Anti-UBE2E3 antibody [OTI4B4] (ab128098)

    HepG2 (human liver hepatocellular carcinoma cell line) cells stained for UBE2E3 using ab128098 at a 1/100 dilution in ICC/IF.

  • Flow Cytometry - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
    Flow Cytometry - Anti-UBE2E3 antibody [OTI4B4] (ab128098)

    Flow cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, using anti-UBE2E3 antibody (ab128098) in Red at 1/100 dilution, compared to a nonspecific negative control antibody in Blue.

  • Flow Cytometry - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
    Flow Cytometry - Anti-UBE2E3 antibody [OTI4B4] (ab128098)

    Flow cytometry analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, using anti-UBE2E3 antibody (ab128098) in Red at 1/100 dilution, compared to a nonspecific negative control antibody in Blue.

  • Flow Cytometry - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
    Flow Cytometry - Anti-UBE2E3 antibody [OTI4B4] (ab128098)

    Flow cytometry analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either UBE2E3 overexpressing plasmid (Red) or empty vector control plasmid (Blue) and immunostained by anti-UBE2E3 antibody (ab128098) at 1/100 dilution.

  • Western blot - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
    Western blot - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
    All lanes : Anti-UBE2E3 antibody [OTI4B4] (ab128098) at 1/2000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cells were transfected with pCMV6-ENTRY UBE2E3 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 22 kDa

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