Anti-USP3 antibody (ab229348)
Key features and details
- Rabbit polyclonal to USP3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP3 antibody
See all USP3 primary antibodies -
Description
Rabbit polyclonal to USP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey
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Immunogen
Recombinant fragment within Human USP3 (internal sequence). The exact sequence is proprietary.
Database link: Q9Y6I4 -
Positive control
- ICC/IF: K562 cells. WB: HEK-293T, A431 and HeLa whole cell lysate; USP3-transfected HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-USP3 antibody (ab229348)
K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for USP3 (green) using ab229348 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Western blot - Anti-USP3 antibody (ab229348)All lanes : Anti-USP3 antibody (ab229348) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa7.5 % SDS-PAGE gel.
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Western blot - Anti-USP3 antibody (ab229348)All lanes : Anti-USP3 antibody (ab229348) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : Human USP3-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa7.5 % SDS-PAGE gel.
