Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP3 antibody (ab101473)

Key features and details

  • Rabbit polyclonal to USP3
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab101473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Horse
Chicken
Dog
Pig
Macaque monkey
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

Target

  • Function

    Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.

  • Tissue specificity

    Expressed in all tissues examined, with strongest expression in pancreas.

  • Sequence similarities

    Belongs to the peptidase C19 family. USP3 subfamily.
    Contains 1 UBP-type zinc finger.

  • Domain

    Both protease activity and an intact zinc finger are required for H2A monodeubiquitination.

  • Cellular localization

    Nucleus. Localizes preferentially with monoubiquitinated H2A to chromatin.
  • Information by UniProt

  • Database links

    • Alternative names

      • Deubiquitinating enzyme 3 antibody
      • EC 3.1.2.15 antibody
      • SIH003 antibody
      • Ubiquitin carboxyl-terminal hydrolase 3 antibody
      • Ubiquitin specific peptidase 3 antibody
      • Ubiquitin specific processing protease 3 antibody
      • Ubiquitin Specific Protease 3 antibody
      • Ubiquitin thioesterase 3 antibody
      • Ubiquitin thiolesterase 3 antibody
      • Ubiquitin-specific-processing protease 3 antibody
      • UBP antibody
      • UBP3_HUMAN antibody
      • Usp3 antibody
      see all

    Images

    • Western blot - Anti-USP3 antibody (ab101473)
      Western blot - Anti-USP3 antibody (ab101473)
      All lanes : Anti-USP3 antibody (ab101473) at 1 µg/ml

      Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
      Lane 2 : : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 59 kDa
      Observed band size: 59 kDa
      Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes

    References (2)

    Publishing research using ab101473? Please let us know so that we can cite the reference in this datasheet.

    ab101473 has been referenced in 2 publications.

    • Zhou Q  et al. Ubiquitin-specific protease 3 targets TRAF6 for deubiquitination and suppresses IL-1ß induced chondrocyte apoptosis. Biochem Biophys Res Commun 514:482-489 (2019). PubMed: 31056254
    • Wang Z  et al. Downregulated USP3 mRNA functions as a competitive endogenous RNA of SMAD4 by sponging miR-224 and promotes metastasis in colorectal cancer. Sci Rep 7:4281 (2017). WB ; Human . PubMed: 28655924

    Images

    • Western blot - Anti-USP3 antibody (ab101473)
      Western blot - Anti-USP3 antibody (ab101473)
      All lanes : Anti-USP3 antibody (ab101473) at 1 µg/ml

      Lane 1 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
      Lane 2 : : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 59 kDa
      Observed band size: 59 kDa
      Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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