Anti-BCAR1 (phospho Y762) antibody (ab41827)
Key features and details
- Rabbit polyclonal to BCAR1 (phospho Y762)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BCAR1 (phospho Y762) antibody
See all BCAR1 primary antibodies -
Description
Rabbit polyclonal to BCAR1 (phospho Y762) -
Host species
Rabbit -
Specificity
This antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated BCAR1 on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 762 in the Src binding domain of rat BCAR1. This rat sequence is the same as Tyr-664 in human BCAR1 and Tyr-664 in mouse BCAR1. This site is also highly conserved in Cas-L (HEF1, Nedd9).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-BCAR1(Tyr-751) peptide before affinity purification using phospho-BCAR1(Tyr-762) peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BCAR1 (phospho Y762) antibody (ab41827)Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4-6). The blot was probed with anti-BCAR1 (lanes 1 & 2) or anti-BCAR1 (Tyr-762) (lanes 3-6). The latter was used in the presence of no peptide (lane 4), phospho-p130 Cas (Tyr-762) peptide (lane 5) or phospho-BCAR1 (Tyr-751) peptide (lane 6).
